Sample preparation
Instruments for carbon coating, plasma cleaning, high-pressure freezing, freeze substation, (cryo-)ultramicrotomy and vitrification
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Microscopy
Three transmission electron microscopes (TEMs), one scanning electron microscope (SEM), access to two advanced cryo-electron microscopes at the NeCEN facility. Some of our TEMs are equipped with an integrated light microscope for CLEM, HAADF STEM and energy filters for imaging thick samples. Hardware and software for automated 2D and 3D data collection is available. The SEM has an integrated sectioning knife for large 3D volume imaging (Serial Block Face SEM). We have two dedicated cryo-light microscopy setups for (super-resolution) cryo-CLEM.
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Computing and visualization
Our computing infrastructure includes Windows and Linux workstations on which EM related software is implemented. We have dedicated hardware for GPU-based computations. We are connected to the LUMC in-house computing and data storage infrastructure.
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NeCEN
The Netherlands Center for Electron Nanoscopy (NeCEN) is the Dutch advanced cryo-electron microscopy facility situated in Leiden at the Institute Biology Leiden (IBL) at Leiden University (UL). It specializes in data collection for high-resolution structure determination of single particle analysis of proteins and viruses and in data acquisition for cryo-electron tomography of protein complexes and bacteria.
